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Products

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension can be used to pack your own columns for protein purification. 50ml Strep-Tactin XT Superflow 50% suspension

IBA GmbH™ 10x Buffer BXT; Strep-Tactin™XT Elution B.

IBA GmbH™ 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT. 25ML 10x Buffer BXT; Strep-TactinXT Elution Buffer

IBA Solutions For Life Science™ D-Desthiobiotin

Used for elution of Strep-tag™ II proteins from Strep-Tactin™ D-Desthiobiotin, 5 g, 5g

IBA Solutions for Life Science™ StrepMAB-Classic

For detection of N- or C-terminal or internal Strep-tag™ II specific monoclonal antibody 100UG STREPMAB-CLASSIC PURIFIED LYOPHILIZEDpurified, 100 µg, 100µg

IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension

Ideal for gravity flow purification of recombinant Strep-tag™ proteins. IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension is conveniently formulated as a 50% Sepharose™ suspension. 20 ML Strep-Tactin(R) Sepharose 50 % suspension

IBA Lifesciences™ 5mL Gravity Flow Column

5 ML GRAVITY FLOW STREP-TACTIN(R) SEPHAROSE COLUMN, 5 mL 00 Store in 4C Fridge

IBA Solutions for Life Science™ Strep-tag™ Regeneration Buffer, 10X

Strep-tag® regeneration bufferwith HABA, 100 ml, 100ml

IBA Lifesciences™ Strep-Tactin Sepharose

50 ML STREP-TACTIN(R) SEPHAROSE 50 % SUSPENSION50mL Store in 4C Fridge

IBA Solutions For Life Science™ UpC Dinucleotide

1 MG UpC-dinucleotide 1mg Store in Freezer at -20C

IBA GmbH™ GFP-Twin-Strep-tag Control Protein

IBA GmbH™ GFP-Twin-Strep-tag Control Protein is for test purifications with Strep-Tactin™ and Strep-Tactin™ XT. GFP-Twin-Strep-tag control protein

IBA GmbH™ Strep-Tactin™XT Coated Microplate

Strep-Tactin™XT Coated Microplate provides efficient capturing of Twin-Strep-tag™ fusion proteins in assays with pM affinity. Strep-Tactin XT coated microplate

IBA Solutions For Life Science™ UpC-dinucleotide IBA

1 MMOL UpC-dinucleotide 1µmol Store in Freezer at

IBA Lifesciences™ Conjugat Strep-Tactin-HRP

Used for fast and direct detection of Strep-tag™II fusion proteins transferred to a blotting membrane or coated to a microplate. IBA Lifesciences™ Strep-Tactin-HRP Conjugate is suitable for chromogenic and chemiluminescence reactions. Strep-Tactin® HRP conjugate,0.5 ml, 0.5ml

IBA Solutions For Life Science™ UpU-dinucleotide IBA

5 MG UpU-dinucleotide 5mg Store in Freezer at -20C

IBA Solutions For Life Science StarGate™ pLSG-IBA Acceptor Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination. pLSG-IBA25; 5µg

IBA Solutions For Life Science™ 4-Thio-UTP

500 NMOL 4-Thio-UTP 0.5µmol Store in Freezer at-20°C

IBA Solutions For Life Science StarGate™ pESG-IBA Acceptor Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen. pESG-IBA43; 5µg

IBA Solutions For Life Science™ 7-Deaza-GTP IBA

500 NMOL 7-Deaza-GTP 0.5µmol Store in Freezer at

IBA Solutions For Life Science 2'-Amino-2'-dUTP

A modified nucleoside-triphosphate (3´-modified oligos) that prevents nuclease degradation of RNAs. 2'-Amino-2'-dUTP

IBA Solutions For Life Science Hexynyl-ApG Transcription Starter

A modified RNA oligonucleotide for 5´-labeled transcripts that allows click reaction with a number of azide labels and can be used for in vitro transcription experiments. Hexynyl-ApG

IBA GmbH™ Mammalian Expression Vector with No Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA GmbH™ Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBA145; 5µg

IBA GmbH™ CD4+ CD25+ CD45RA+ Treg Fab Streptamer™ Isolation Kit MB

IBA GmbH™ CD4+ CD25+ CD45RA+ Treg Fab Streptamer™ Isolation Kit MB is for reversible cell isolation delivering label-free target cells. CD4+ CD25+ CD45RA+ Treg Fab Streptamer Isolation Kit MB

IBA Solutions For Life Science StarGate™ pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins. pPSG-IBA43; 5µg

IBA Solutions For Life Science™ GpC-dinucleotide IBA

5 MG GpC-dinucleotide 5mg Store in Freezer at -20C

IBA Solutions For Life Science™ GpC-dinucleotide IBA

10 MG GpC-dinucleotide 10mg Store in Freezer at

IBA GmbH™ HA-1H peptide VLHDDLLEA (HLA-A*0201)

IBA GmbH™ HA-1H peptide VLHDDLLEA (HLA-A*0201) is a single peptide for stimulation of human HA-1H - specific CD8+ T-cells. 1MG HA-1H peptide VLHDDLLEA (HLA-A*0201)

IBA Solutions For Life Science C8-Alkyne-dCpA-PH Transcription Starter

A DNA/RNA oligonucleotide for 5´-labeled transcripts that allows click reaction with a number of azide labels. C8-Alkyne-dCpA-PH

IBA Solutions For Life Science™ 3'-O-Methyl-ATP IBA

500 NMOL 3'-O-Methyl-ATP 0.5µmol Store in Freezer

IBA GmbH™ E. coli Expression Vector with No Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA GmbH™ E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli. pASG-IBA145; 5µg

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Featured Products

Twin-Strep-tag™/Strep-Tactin™XT


Twin-Strep-tag

The purification system with the highest affinity – one tag for expression, purification, detection and immobilization

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Immobilization with near covalent binding affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR experiments »

  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated

High affinity meets high capacity!


High affinity meets high capacity!

Strep-Tactin™XT Superflow™ high capacity »

  • Higher protein yields compared to all other Strep-Tactin™ resins
  • Dynamic binding capacity of ~15 mg protein per ml resin
  • Elution of concentrated protein due to small elution volumes
  • Cost efficent: small price for more protein

Try our new high affinity Strep-tag™ system


Try our new high affinity Strep-tag® system

Starter Kit consists of:

  • Strep-Tactin™XT Superflow™ gravity flow column
  • Buffer W (Strep-Tactin™/Strep-Tactin™XT Wash Buffer)
  • Buffer BXT (Strep-Tactin™XT Elution Buffer with Biotin)
  • Strep-Tactin™ conjugated with HRP (for Western blot detection)
  • Short protocol
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free cells
  • Directly from whole blood
  • No centrifugation

Fab-TACS™ Gravity and FABian™ »

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™ »

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™ »

  • Completely reversible reagents
  • Detachment from the cells at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Non-magnetic and bead-free

Peptides for T cell stimulation »

  • Purity > 80 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ »

Detection of receptor-specific T cells with Fab Streptamers™ »

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning & Transfection


Cloning & Transfection

More than 150 expression vectors »

  • Variety of affinity tags
  • Expression hosts:  E. coli, mammalia, insect cells, yeast
  • StarGate cloning system

Mammalian Expression


The MEXi system »

Optimized for efficient protein expression and Strep-tag™ purification in mammalian cells.

Protein Purification


Protein Purification

Strep-tag™ system from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA »

  • Strep-Tactin™ conjugates for Strep-tag™II/Twin-Strep-tag™ proteins
  • Monoclonal antibodies against Strep-tag™II/Twin-Strep-tag™

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin™XT:Twin-Strep-tag™ for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore)

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated

Immunoprecipitation


Selector resins based on high-affinity single-domain antibodies (sdAb)

  • High capacity
  • Minimal unspecific binding
Oligos
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DNA


RNA


DNA & RNA

Standard DNA oligonucleotides

  • Long range up to 200 bases*
  • Large scale oligonucleotides
  • 3’-, 5’- or internally modified

Random RNA, dsRNA, siRNA, miRNA

  • Long range up to 100 bases*
  • 3’-, 5’- or internally modified
  • 2‘-modified: -O-Me, -F, -NH2
  • Nucleoside Triphosphates
  • RNA and RNA/DNA hybrid
  • Dinucleotides

*depending on sequence and scale

Fluorescence Labels


Non-Fluorescence Labels


Fluorescence Labels

E.g. for FRET, qPCR, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami

  • For dual and multiply labeled DNA and RNA
  • Excellent high quality 
  • More than 200 labels to choose from
  • Quenchers such as BBQ and BHQ™
  • Completely new dye combinations available

For DNA microarrays, surface binding and hybridization experiments, cross-linking and crystallography studies and more

  • More than 80 internal, 5’- and 3’- DNA and RNA modifications 

Backbone Modifications


Aptamers


Backbone Modifications
  • Phosphorothioates

Custom synthesis of DNA and RNA aptamers

  • High affinity 
  • Low immunogenicity
Resources Strep-Tactin®XT Sample Request
IBA_about_us

About IBA GmbH

Solutions For Life Sciences

IBA GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.