BrdU Monoclonal Antibody (MoBU-1), Invitrogen™

Description

The MoBU-1 clone has a high specificity for BrdU, in addition to no cross-reactivity with EdU. This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay. Samples labeled with the Alexa Fluor™ conjugates of MoBU-1 can be directly imaged by fluorescence microscopy or high-content imaging. The unconjugated form and the biotin conjugate of this clone require a secondary antibody or a streptavidin conjugate for detection, respectively. The MoBU-1 clone is also available for flow cytometry applications.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

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