Learn More
CD123 Monoclonal Antibody (6H6), Super Bright™ 645, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marque: Affymetrix eBioscience 64-1239-42
Informations supplémentaires : Poids : 0.01000kg
Description
Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127. Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis. Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
CD123 Monoclonal antibody specifically detects CD123 in Human samples. It is validated for Flow Cytometry.Spécification
CD123 | |
Monoclonal | |
5 μL/Test | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
P26951 | |
IL3RA | |
Affinity chromatography | |
RUO | |
3563 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
6H6 | |
Super Bright 645 | |
IL3RA | |
CD123; CD123 antigen; CDw123; Cyrl; cytokine receptor-like protein CYRL; hIL-3Ra; Il-3 alpha subunit; IL-3 receptor alpha chain; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL3R; IL-3R subunit alpha; Il3ra; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor subunit alpha; interleukin 3 receptor, alpha; interleukin 3 receptor, alpha (low affinity); interleukin 3 receptor, alpha chain; interleukin-3 receptor class II alpha chain; Interleukin-3 receptor subunit alpha; Sut-1 | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |