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CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Super Bright™ 600, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Marque:  Affymetrix eBioscience 63-1541-82

242.01 EUR valable jusqu'au 2024-03-29
Utilisez le code promo "21615" pour bénéficier de cette offre.



Informations supplémentaires : Poids : 0.01000kg

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Code produit. 15973534

  • 363.00€ / 100µg
Expédition estimée: 16-04-2024
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Description

Description

Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle. For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1. Applications Reported: This MR1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter.

CD154 (CD40 Ligand) Monoclonal antibody specifically detects CD154 (CD40 Ligand) in Mouse samples. It is validated for Flow Cytometry.
Spécification

Spécification

CD154 (CD40 Ligand)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P27548
CD40LG
Affinity chromatography
RUO
21947
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
MR1
Super Bright 600
CD40LG
CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5
Armenian Hamster
100 μg
Primary
Mouse
Antibody
IgG
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