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CD162 (PSGL-1) Mouse anti-Human, Super Bright 436, Clone: FLEG, eBioscience™

Mouse Monoclonal Antibody

Marque:  Invitrogen 62-1629-41

Informations supplémentaires : Poids : 0.01000kg

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Code produit. 15875513

  • 180.00€ / 25 pièces
Date d’expédition estimée: 13-12-2021

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Description

Description

Description: This FLEG monoclonal antibody recognizes human CD162, which is also known as P-selectin glyocoprotein ligand-1 (PSGL-1). This 120-kDa protein exists as a homodimer on the surface of monocytes, neutrophils, granulocytes, peripheral T cells, some B cells, and a subset of CD34+ hematopoietic progenitor cells (HPCs) in the bone marrow. CD162 binds CD62P (P-selectin), CD62E (E-selectin), and CD62L (L-selectin) to mediate leukocyte interactions with each other as well as with activated platelets and endothelium. Thus, CD162 plays a critical role in leukocyte adhesion and rolling. Furthermore, the binding of CD162 to P-selectin on HPCs has been demonstrated to inhibit hematopoiesis. Studies have also demonstrated CD162 as the receptor for enterovirus 71. Applications Reported: This FLEG antibody has been reported for use in flow cytometric analysis. Applications Tested: This FLEG antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent.

Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.
Spécification

Spécification

CD162 (PSGL-1)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
Q14242
PSGL1, PSGL-1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
FLEG
Super Bright 436
SELPLG
Liquid
SELPLG
IgG2a, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
6404
Vidéos
FDS
Documents
Certificats
Promotions

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For Research Use Only