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CD272 (BTLA) Monoclonal Antibody (8F4), APC, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marque: Affymetrix eBioscience 17-5956-82
Code nomenclature Nacres: NA.41
Informations supplémentaires : Poids : 0.00001kg
Description
Description: The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction. Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes. It is not understood why there is a discrepancy in thymocyte staining. However, in staining of splenocytes and bone marrow cells, clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7. Applications Reported: This 8F4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 8F4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
CD272 (BTLA) Monoclonal antibody specifically detects CD272 (BTLA) in Mouse samples. It is validated for Flow Cytometry.Spécification
| CD272 (BTLA) | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Q7TSA3 | |
| BTLA | |
| Affinity chromatography | |
| RUO | |
| 208154 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| 8F4 | |
| APC | |
| BTLA | |
| A630002H24; B and T lymphocyte associated; B and T lymphocyte associated protein; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA_HUMAN; BTLA1; CD272; CD272 antigen; FLJ16065; MGC129743 | |
| Mouse | |
| 100 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG1 κ |
For Research Use Only.