CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright™ 702, eBioscience™, Invitrogen™

Description

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.