CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Super Bright™ 702, eBioscience™, Invitrogen™

Description

Description: The CD66a-B1.1 monoclonal antibody reacts with human CD66a, c, d, and e, which are also known as CEACAM1, CEACAM6, CEACAM3 and CEA, respectively. CD66 is expressed on endothelial and epithelial cells, as well as on neutrophils and monocytes. Myeloma and cancer cells also express CD66. Moreover, expression of this receptor can be induced on T cells, B cells, and CD16-negative NK cells. CD66 exists as multiple splice variants that differ in the length of their cytoplasmic domain, which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that mediates the inhibitory functions of this cell surface protein on T and NK cells. CD66 is involved in cell adhesion and growth, neutrophil activation, and signaling. Finally, studies have shown that the CD66 variants interact with each other homo- and heterotypically. Applications Reported: This CD66a-B1.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CD66a-B1.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm.