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CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Marque: Affymetrix eBioscience 46-0083-82
Code nomenclature Nacres: NA.41
Informations supplémentaires : Poids : 0.25000kg
Description
Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells. Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat.
CD8b Monoclonal antibody specifically detects CD8b in Mouse samples. It is validated for Flow Cytometry.Spécification
| CD8b | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| P10300 | |
| Cd8b1 | |
| Affinity chromatography | |
| RUO | |
| 12526 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| eBioH35-17.2 (H35-17.2) | |
| PerCP-eFluor 710 | |
| Cd8b1 | |
| CD8; CD8 antigen 37 kDa chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8a; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; CD8B1; CD8beta; CD8BP; LEU2; Leu-2; LY3; Ly-3; Ly-C; Lymphocyte antigen 3; Lyt3; Lyt-3; MAL; OX-8 membrane antigen; p32; P37; T lymphocyte surface glycoprotein beta chain; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3 | |
| Rat | |
| 100 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2b κ |
For Research Use Only.