missing translation for 'onlineSavingsMsg'
Learn More

FOXP3 Rat anti-Bovine, Canine, Feline, Mouse, Porcine, Rat, eFluor® 450, Clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 48-5773-82

Code nomenclature Nacres: NA.41

Informations supplémentaires : Poids : 0.25000kg

 Afficher plus de versions de ce produit

Code produit. 15331790

  • 334.00€ / 100µg

En stock, prêt à être expédié : 9

Veuillez vous pour pouvoir commander cet article. Besoin d'un compte web? Créer le vôtre dès maintenant!


Description

Description

Applications Tested: This FJK-16s antibody has been tested by intracellular staining using Foxp3 buffers of mouse splenocytesusing Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered. FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
Spécification

Spécification

Foxp3
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q99JB6
AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Bovine, Canine, Feline, Mouse, Porcine, Rat
Flow Cytometry
FJK-16s
eFluor™ 450
Foxp3
Liquid
FOXP3
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
100037405, 20371, 317382, 444998, 491876, 506053
Vidéos
Documents

For Research Use Only.