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Thermo Scientific™ GeneBLAzer™ M4-Gqo5-NFAT-bla CHO-K1 Cells
GeneBLAzer™ M4 Gqo5-NFAT-bla CHO-K1 cells contain the human Acetylcholine (muscarinic) subtype 4 receptor (M4) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line.
Marque: Thermo Scientific™ K1741
Description
GeneBLAzer™ M4 Gqo5-NFAT-bla CHO-K1 cells contain the human Acetylcholine (muscarinic) subtype 4 receptor (M4) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cells (Cat. No. K1536) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Chimeric G protein, Gqo5.
The GeneBLAzer™ M4 Gqo5-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Cabachol. In addition, GeneBLAzer™ M4 Gqo5-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
The clinical implications of the M4 receptor are unknown, however studies with knockout mice suggest that M4 may have implications in dopaminergic function, karatinocyte migration and wound healing, anxiolysis, and analgesia. In addition, M4 may be important in memory circuits and have implications in Alzheimer's Disease. Additional information on the muscarinic receptors can be found in literature.
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Spécification
NM_000741 | |
Division cellulaire | |
État de la population : Origine de clones à cellule unique | |
FRET | |
Cellules GeneBLAzer™ M4 Gqo5 CHO-K1 DA, protocole de dosage GeneBLAzer™ M4 Gqo5 CHO-K1 DA, certificat d’analyse, solution D, protocole de chargement LiveBLAzer™-FRET B⁄G | |
GeneBLAzer™ | |
BLA (bêta-lactamase) | |
Division cellulaire | |
GeneBLAzer™ |
Gène rapporteur de la bêta-lactamase cellulaire | |
Contenu : Cellules K1741 DA CHO-K1 M4 Gqo5 GeneBLAzer™ (Kit de chargement LiveBLAzer™-FRET B⁄G, solution D et autres matériaux requis séparément ; reportez-vous au protocole). Comprend : • Cellules K1741 DA CHO-K1 M4 Gqo5 GeneBLAzer™ • Protocole de dosage DA CHO-K1 M4 Gqo5 GeneBLAzer™ • Certificat d’analyse • Solution D • Protocole de chargement LiveBLAzer™-FRET B⁄G Stockage : Les cellules CHO-K1 M4-Gqo5-NFAT-bla GeneBLAzer™ sont expédiées sur de la glace sèche et doivent être stockées immédiatement dans de l’azote liquide. |
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Fluorescent | |
M4 | |
Origine de clones à cellule unique | |
2 x 106 cellules | |
M4 | |
CHO-K1 | |
GPCR |
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Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.