Invitrogen™ Human IL-4, Recombinant Protein

Description

Interleukin-4 (IL-4) is a pleiotropic,immune-modulatory cytokine produced by activated T cells,and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13,which may contribute to many overlapping functions of this cytokine and IL13. STAT6,a signal transducer and activator of transcription,has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene,IL-3,IL-5,IL-13,and CSF2 form a cytokine gene cluster on chromosome 5q. In particular,IL-4 is located in close proximity to IL13 on the chromosome. Further,the IL-4 gene,IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4. Mature human IL-4 was expressed in Pichia pastoris,X-33. GenBank Accession # P05112,amino acids 25 – 153. For protein verification,the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLQEI. Purity is >95% by SDS-PAGE analyses. Activity: The biological activity of recombinant human IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line,TF-1. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4. Mature human IL-4 was expressed in Pichia pastoris, X-33. GenBank Accession &Num; P05112, amino acids 25 – 153. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLQEI. Purity is >95% by SDS-PAGE analyses. Activity: The biological activity of recombinant human IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Store lyophilized material below 0°C. Reconstitute in distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.