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IL-12/IL-23 p40 Rat anti-Mouse, Biotin, Clone: C17.8, eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 13-7123-85

Code nomenclature Nacres: NA.41

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15340040

  • 264.00€ / 500µg

Expédition estimée
27-10-2020

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Description

Description

For mouse IL-23 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-23 protein levels, in combination with the purified G23-8 antibody (14-7232) for capture and recombit mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25 - 1 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 4,000 pg/mL - 30 pg/mL should be included in each ELISA plate. Note: TMB (00-4201), rather than ABTS, should be used as the substrate. For mouse IL-12 p40 Total ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse total p40 (e.g., p40 monomer, p40 dimer, p40/p35, and p40/p19), in combination with the purified C15.6 antibody (14-7125) for capture and recombit mouse IL-12 p40 (39-8122), IL-12 p70 (39-8121) or mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates. Filtration: 0.2 μm post-manufacturing filtered. IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintece and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
Spécification

Spécification

IL-12/IL-23 p40
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P43432
IL12B; Il12p40; interleukin 12B; RP23-388G23.1; Il-12b; Il-12p40; p40; IL-12 p40; IL-23 subunit p40; IL12P40
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
ELISA
C17.8
Biotin
Il12b
Liquid
Il12b
IgG2a, kappa
500 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16160
Vidéos
Documents

For Research Use Only.