Ki-67 Monoclonal Antibody (SolA15), eFluor™ 570, eBioscience™, Invitrogen™

Description

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the ""Ki-67 labeling index"" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. Applications Reported: This SolA15 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This SolA15 antibody has been tested immunocytochemistry on fixed and permeabilized C2C12 cells and can be used at less than or equal to 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Ki-67 Monoclonal antibody specifically detects Ki-67 in Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).