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Ly-6C Monoclonal Antibody (HK1.4), APC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Marque: Affymetrix eBioscience 17-5932-80
Code nomenclature Nacres: NA.41
Informations supplémentaires : Poids : 0.25000kg
Description
Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells. In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release. Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
Ly-6C Monoclonal antibody specifically detects Ly-6C in Mouse samples. It is validated for Flow Cytometry.Spécification
| Ly-6C | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| P0CW02 | |
| Ly6c1 | |
| Affinity chromatography | |
| RUO | |
| 17067 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| HK1.4 | |
| APC | |
| Ly6c1 | |
| AA682074; AA959465; Ly6c; Ly-6C; Ly6c1; Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C | |
| Rat | |
| 25 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2c κ |
For Research Use Only.