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MCL-1 Monoclonal Antibody (LVUBKM), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

155.00€ - 354.00€

Spécification

Antigène MCL-1
Clone LVUBKM
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
Consulter d'autres spécifications

Produits 2
Code produit Marque Quantité Prix Quantité et disponibilité  
Code produit Marque Quantité Prix Quantité et disponibilité  
16360634
Afficher les documents Détails de la promotion
Affymetrix eBioscience
12-9047-41
25 Tests
155.00€
25 pièces
Voir les produits de remplacement
Économisez jusqu'à 
»
Expédition estimée: 22-04-2024
pour voir le stock disponible 
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16370634
Afficher les documents Détails de la promotion
Affymetrix eBioscience
12-9047-42
100 Tests
354.00€
100 pièces
Voir les produits de remplacement
Économisez jusqu'à 
»
Expédition estimée: 22-04-2024
pour voir le stock disponible 
Veuillez vous pour pouvoir commander cet article. Besoin d'un compte web? Créer le vôtre dès maintenant!
Description

Description

Description: This LVUBKM monoclonal antibody recognizes human and mouse myeloid cell leukemia sequence 1 (Mcl-1). Mcl-1 is an anti-apoptotic member of the Bcl-2 family of proteins important for regulation of cell survival/apoptosis. Mcl-1 is primarily localized to the outer membrane of mitochondria where it prevents cytochrome c release via dimerization with other Bcl-2 family members such as Bim. Although it is expressed in both immune and non-immune cells, highest levels of Mcl-1 expression are seen in hematopoietic lineage cells. PI3K activation of AKT results in destabilization and degradation of GSK3 beta, which prevents phosphorylation of Mcl-1 on S159 and its subsequent ubiquitnation and degradation. Mice conditionally lacking Mcl-1 in lymphocytes showed that Mcl-1 is essential during early lymphoid development and for the maintenance of mature lymphocytes. Applications Reported: This LVUBKM antibody has been reported for use in flow cytometric analysis. Applications Tested: This LVUBKM antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, μgtraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.
Spécification

Spécification

MCL-1
5 μL/Test
Monoclonal
Liquid
RUO
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
AW556805; BCL2 family apoptosis regulator; BCL2L3; bcl2-L-3; Bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; EAT; Induced myeloid leukemia cell differentiation protein Mcl-1; induced myeloid leukemia cell differentiation protein Mcl-1 homolog; Mcl1; Mcl-1; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; MCL-1S; MGC104264; MGC1839; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1; myeloid cell leukemia sequence 1 (BCL2-related); TM
MCL1
Primary
4° C, store in dark, DO NOT FREEZE!
MCL1
LVUBKM
Flow Cytometry
PE
Mouse
Human, Mouse
P97287, Q07820
17210, 4170
IgG1 κ
Affinity chromatography
Antibody
Vidéos
FDS
Documents

Documents

Product Certifications