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MHC Class I (H-2Db) Mouse anti-Mouse, PE-Cyanine7, Clone: 28-14-8, eBioscience™ Invitrogen™

Mouse Monoclonal Antibody

Marque:  Invitrogen 25-5999-80

Informations supplémentaires : Poids : 0.01000kg

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Code produit. 16646324

  • 220.00€ / 25µg

Date d’expédition estimée: 04-11-2021

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The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin. Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

MHC Class I (H-2Db) Monoclonal antibody specifically detects MHC Class I (H-2Db) in Mouse samples. It is validated for Flow Cytometry


MHC Class I (H-2Db)
Flow Cytometry
H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P01895, P01896, P01897, P01899, P01900, P14426, P14427
Affinity chromatography