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GFP Polyclonal Antibody, Alexa Fluor™ 555, Invitrogen™

Rabbit Polyclonal Antibody

Marque:  Invitrogen A31851

356.01 EUR valable jusqu'au 2024-03-29
Utilisez le code promo "21615" pour bénéficier de cette offre.



Code nomenclature Nacres: NA.41

Informations supplémentaires : Poids : 0.00450kg

Code produit. 10719194

  • 534.00€ / 1 pièce
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Description

Description

At the time of preparation, the products are certified to be free of unconjugated dyes and are tested in a cytological experiment to ensure low nonspecific staining. Several Alexa Fluor™ dye-conjugates made from the rabbit anti-GFP IgG fraction are also available. The Alexa Fluor™ dyes provide for extraordinarily bright antibody conjugates. The approximate fluorescence excitation (Ex) and emission maxima for this Alexa Fluor™ 555 conjugate are 555 nm and 565 nm, respectively. These anti-GFP antibody conjugates are suited for detection of native GFP, GFP variants, and most GFP-fusion proteins by western blot analysis and immunocytochemistry. For initial experiments, we recommend trying dilutions ranging from 1:200 to 1:2,000 with our fluorophore-labeled antibodies, a final concentration of 1-10 μg/mL should be satisfactory for most immunocytochemical applications. If using the rabbit anti-GFP antibody-Alexa Fluor™ conjugates in western blotting, dilute the conjugate 1:2,000 in PBS to obtain a final antibody concentration of 1 μg/mL. HeLa and U2-OS cells were tested with this antibody in immunocytochemistry, but not with paraffin-embedded sections. It is a good practice to centrifuge the protein conjugate solutions briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step eliminates any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

GFP Polyclonal antibody specifically detects GFP in Tag samples. It is validated for ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot.

Informations sur la commande

Shipping Condition: Wet Ice

Spécification

Spécification

GFP
Polyclonal
Alexa Fluor 555
GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
The GFP was isolated directly from the jellyfish Aequorea victoria.
100 μL
Primary
Antibody
IgG
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
2 mg/mL
PBS with 5mM sodium azide; pH 7.5
Rabbit
Ion-exchange chromatography
RUO
4° C, store in dark
Liquid
Tag
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For Research Use Only. Not for use in diagnostic procedures.