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Thermo Scientific™ Kit d‘isolement de glycoprotéine WGA et ConA Pierce™

Purifiez les glycoprotéines à partir des lysats cellulaires et sériques à l’aide de résines d’affinité ou de kits avec des lectines de concanaviline A (ConA) ou d’agglutinine de germe de blé (WGA).

Marque:  Thermo Scientific™ 89804

Code nomenclature Nacres: NA.32

Informations supplémentaires : Poids : 0.10000kg Transport : UN number : 3261 Chem class : 8 Pack group : III

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Code produit. 10007003

  • 313.00€ / 1 kit


Stock EU 4
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Description

Description

These two kits use lectins concanavilin A (ConA) and wheat germ agglutinin (WGA), respectively, as ligands to bind specific classes of glycoproteins from complex protein samples. ConA lectin recognizes alpha-linked mannose and terminal glucose residues, while WGA lectin selectively binds to N-Acetyl glucosamine (GlcNAc) groups and to sialic acid. The kits include ConA or WGA beaded agarose resin, optimized binding/wash and elution buffers, and easy-to-use microcentrifuge spin columns. The kits have been used to successfully isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.

Highlights:

  • High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers

  • Fast – glycoprotein purification in less than one hour

  • Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate

  • Robust – lectin does not leach from resin when processing sample

  • Convenient – complete kits include lectin resins and spin columns with all necessary reagents

  • Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Includes:

ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.

Spécification

Spécification

Kit d‘isolement de glycoprotéine Pierce, ConA
Résine de lectine Cona, 1,1 ml, Tubes de prélèvement, 20 tubes, Tampon d’élution, 5 ml, Colonnes à centrifuger, 10 colonnes, Tampon de liaison/lavage (5 X), 6,5 ml
Isolement de glycoprotéines ayant une affinité forte pour la ConA à partir de 10 échantillons d‘un volume allant jusqu‘à 640 µl (de 1 à 1,5 mg de protéine totale) chacun
Kit multicomposants avec de la résine d’agarose de concanavaline A
Kit pour 10 réactions
Agarose
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