missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ Pierce™ SILAC Protein Quantitation Kits
Analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) using these complete reagent kits.
652.00€ - 661.50€
Spécification
| Suffisant pour | One complete SILAC experiment (stable isotope labeling with amino acids in cell culture) |
|---|---|
| Quantité | 1 Use Kit |
| À utiliser avec (application) | SILAC experiments (stable isotope labeling with amino acids in cell culture) |
| Code produit | Marque | Description | Prix | Quantité et disponibilité | |||||
|---|---|---|---|---|---|---|---|---|---|
| Code produit | Marque | Description | Prix | Quantité et disponibilité | |||||
|
11688833
|
Thermo Scientific™
88439 |
SILAC Protein Quantitation Kit - DMEM:F12 |
Cet article est actuellement indisponible ou discontinu.
Veuillez consulter la page produit pour de possible alternative dans l’onglet |
||||||
|
10220204
|
Thermo Scientific™
89982 |
SILAC Protein Quantitation Kit - RPMI 1640 |
Cet article est actuellement indisponible ou discontinu.
Veuillez consulter la page produit pour de possible alternative dans l’onglet |
||||||
Description
Thermo Scientific™ SILAC Protein Quantitation Kits are used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).
SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.Highlights:
Efficient – 100% label incorporation into proteins of living cells
Reproducible – eliminates intra-experimental variability caused by differential sample preparation
Flexible – media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling
Compatible – label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM, RPMI-1640 or DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others
Optimized, specialized media – the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry
High-quality supplements – heavy amino acids with >99% isotope purity; the Stem Cell Screened Dialyzed FBS and Serum Substitute for Mouse Embryonic Stem Cells are tested to ensure that they do not promote stem cell differentiation
Recommended for:
Quantitative analysis of relative changes in protein abundance from different cell treatments; Quantitative analysis of proteins for which there are no antibodies available; Protein expression profiling of normal vs. disease cells; Identification and quantification of hundreds to thousands of proteins in a single experiment
Spécification
| One complete SILAC experiment (stable isotope labeling with amino acids in cell culture) | |
| 1 Use Kit | |
| SILAC experiments (stable isotope labeling with amino acids in cell culture) |