Serpina12 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Vaspin. Anti-Human Vaspin-specific antibody was purified by affinity chromatography employing an immobilized Human Vaspin matrix Sandwich ELISA: To detect Human Vaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Vaspin (500-P256Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Vaspin. Western Blot: To detect Human Vaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Vaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.