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Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit

Optimisé pour la synthèse d’ADNc premier brin directement à partir d’un lysat de cellules de mammifères sans avoir besoin d’isoler l’ARN au préalable. La lyse cellulaire et la transcription inverse sont effectuées dans le même tube et l’ADNc premier brin qui en résulte est prêt à être utilisé dans le clonage, la PCR et la qPCR.

Marque:  Invitrogen™ 11750350

3441.28 EUR valable jusqu'au 2021-12-22
Utilisez le code promo "16346" pour bénéficier de cette offre.



Informations supplémentaires : Poids : 0.10500kg

 Afficher plus de versions de ce produit

Code produit. 16653873

  • 4528.00€ / 1 pièce

Date d’expédition estimée: 17-11-2021

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Description

Description

Features of the SuperScript™ IV kit include:

  • Compatible with a wide range of mammalian cell types grown under different treatment conditions
  • Single-tube format minimizes reagent loss, sample loss, and handling time
  • Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
  • High-quality cDNA generated for use in a variety of applications, including cloning, PCR, and qPCR
  • Simple protocol takes around 37 minutes
  • Compatible with TaqMan™ and SYBR Green qPCR master mixes from various manufacturers
  • Optimized performance for 1–10,000 cells per sample; results equivalent to those from purified RNA

The SuperScript™ IV CellsDirect cDNA Synthesis Kit includes SuperScript™ IV Reverse Transcriptase. Features of this enzyme include:

  • Reduced RNase H activity
  • Higher thermal stability
  • High yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts
  • Superior tolerance to various RT inhibitors

SuperScript™ IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications. It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript™ III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity.

How it works
In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript™ IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.

Spécification

Spécification

Cellules
Fluorescence
Tube
500 réactions
qPCR, RT-PCR
Kit de synthèse d’ADNc
500 réactions
Glace carbonique
RT-PCR (point final), PCR en temps réel
Haut
500 réactions
SuperScript™
SuperMix ou Master Mix
Vidéos
FDS
Documents
Certificats