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T-bet Monoclonal Antibody (eBio4B10 (4B10)), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marque: Affymetrix eBioscience 53-5825-82
Informations supplémentaires : Poids : 0.00100kg
Description
Description: The eBio4B10 monoclonal antibody reacts with mouse, rhesus moneky and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells. Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBio4B10 antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins located at thermofisher.com/flowprotocols. This may be used at less than or equal to 0.25 μg per test.
T-bet Monoclonal antibody specifically detects T-bet in Human, Mouse, Rhesus Monkey samples. It is validated for Flow Cytometry.Spécification
T-bet | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Q9JKD8, Q9UL17 | |
Tbx21 | |
Affinity chromatography | |
RUO | |
30009, 57765, 694044 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
eBio4B10 (4B10) | |
Alexa Fluor 488 | |
Tbx21 | |
HGNC:11599; LOW QUALITY PROTEIN: T-box transcription factor TBX21; Tbet; T-bet; TBLYM; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; TBT1; TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM; transcription factor T-box 21 | |
Mouse | |
100 μg | |
Primary | |
Human, Mouse, Rhesus Monkey | |
Antibody | |
IgG1 κ |