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TCR V alpha 11 Monoclonal Antibody (RR8-1), APC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Marque: Affymetrix eBioscience 17-5800-82
Code nomenclature Nacres: NA.41
Informations supplémentaires : Poids : 0.25000kg
Description
Description: This RR8-1 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 11 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR8-1 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b (e.g., C57BL/6, B10) and d (e.g., DBA/1, DBA/2) haplotypes. V alpha 11+ T cells are absent in strains with the a (e.g., BALB/c, C3H) or c (e.g., SJL) haplotypes of the Tcra gene complex. Finally, V alpha 11 is expressed predominantly on CD4+ T cells. The RR8-1 antibody recognizes the V alpha 11.1 and 11.2 alleles, but not 11.3. This antibody has been reported to stimulate V alpha 11+ T cells. Applications Reported: This RR8-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RR8-1 antibody has been tested by flow cytometric analysis of mouse lymph node. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
TCR V alpha 11 Monoclonal antibody specifically detects TCR V alpha 11 in Mouse samples. It is validated for Flow Cytometry.Spécification
| TCR V alpha 11 | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Rat | |
| 100 μg | |
| Primary | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| RR8-1 | |
| APC | |
| TCR V alpha11; TCRV alpha 11; TCRV alpha11; Va11; Valpha11 | |
| Affinity chromatography | |
| RUO | |
| Mouse | |
| Antibody | |
| IgG2b κ |
For Research Use Only.