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  5. Invitrogen™ Terminal Deoxynucleotidyl Transferase, recombinant 

Invitrogen™ Terminal Deoxynucleotidyl Transferase, recombinant

Product Code. 11578626
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Quantity:
3 x 500 U
500 U
Unit Size:
500 units
Pack of 3
This item is not returnable. View return policy
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical. See MSDS.

Product Code. 11578626

Brand: Invitrogen™ 10533073

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This item is not returnable. View return policy
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical. See MSDS.

Description

Includes

Vial of 5X buffer [500mM potassium cacodylate (pH 7.2), 10mM CoCl2 , 1mM DTT]

Performance and quality tested

Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA.

  • Source: Purified from baculovirus clone of calf thymus TdT
  • Performance and Quality Testing: Endonuclease, 3' and 5' exodeoxyribonuclease, and levels of incorporation tested
  • Unit Definition: One unit incorporates 1nmol dATP into acid-precipitable material in 1 hr. at 37°C, using d(pA)50 as primer
  • Unit Reaction Conditions: 0.2M potassium cacodylate (pH 7.2), 10mM MgO4 C4 H6 , 1mM 2-mercaptoethanol, 0.5mg/mL BSA, 100 flM d(pA)50, 1mM [3H]dATP, and enzyme in 0.15mL for 1 h at 37°C

Cloning; Restriction Enzyme Cloning; Homopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, 32P- or 35S-label; Apoptosis (TUNEL)

Order Info

Shipping Conditions: Approved for shipment on wet or dry ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C.
Enzyme Terminal Deoxynucleotidyl Transferase
Compatible Buffer 5X Buffer, Reaction Buffer
Quantity 3 x 500 U
Product Type TdT

For Research Use Only. Not for use in diagnostic procedures.

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