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  5. Invitrogen™ Human Amyloid beta 40 ELISA Kit 

Invitrogen™ Human Amyloid beta 40 ELISA Kit

Product Code. 10513005
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Quantity:
2 x 96 Tests
96 Tests
Unit Size:
192 tests
96 tests
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Product Code. 10513005

Brand: Invitrogen™ KHB3481

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Description

ELISA

The Human Amyloid Beta 40 (Hu Abeta 40) ELISA quantitates Hu Abeta 40 in human tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF). The assay will exclusively recognize both natural and recombinant Hu Abeta 40. Principle of the method The Human Abeta 40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Beta-amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.

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TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05067
Assay Range 7.8 to 500 pg/mL
Assay Sensitivity <6 pg/mL
Concentration 9.8 to 500 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Cerebrospinal Fluid, Supernatant, Tissue Homogenates
Target Species Human
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 351
Gene Symbol AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP
Incubation Time 4 hr.
Interassay CV 3.8%
Intraassay CV 2.5
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Hu Aβ40 Detection Antibody, Anti-Rabbit IgG HRP (100 X), HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Marker Gene Aβ 40
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cerebrospinal Fluid 50 μL, Supernatant 50 μL, Tissue Homogenate 50 μL
Storage Requirements 2°C to 8°C
Target Amyloid beta 40
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
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For Research Use Only. Not for use in diagnostic procedures.

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