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  5. Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Product Code. 15669364 Shop All Thermo Scientific Products
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Codice prodotto. 15669364 Fornitore Thermo Scientific™ N. del fornitore 34578
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Descrizione

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

  • Sensitivity: picogram to femtogram sensitivity
  • Stability: 8-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 6 to 24 hours
  • Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

TRUSTED_SUSTAINABILITY

Specifica

Product Type Substrate
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line SuperSignal
Quantity 1 L
Conjugate HRP
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Sensitivity Picogram to high femtogram-level
Signal Duration 6 to 24 hr.
Sufficient For 120 Mini-Gel Blots, 10000 cm2 of Membrane
Content And Storage Sufficient for 120 mini-gel blots; 10,000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 500 mL
• SuperSignal West Pico PLUS Stable Peroxide, 500 mL

Store at room temperature.
Shipping Condition Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.

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