Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned (and performance-guaranteed*) TaqMan Assays, eliminating the need to design custom assays. With 72 hours of assembled-reaction benchtop stability, this master mix enables increased productivity through automation. Simplify your workflow by avoiding assay design and minimizing assay optimization: the same assay can be used for both qPCR and digital PCR (dPCR). To use this master mix, pair it with Applied Biosystems predesigned TaqMan Assays, MAP16 plates, and the QuantStudio Absolute Q Digital PCR System.

Features of Absolute Q Universal DNA Digital PCR Master Mix include:

• Universal master mix compatible with a wide range of applications
• Over 20 million predesigned TaqMan Assays available (with a performance guarantee*), eliminating assay design and enabling flexible target selection
• 72-hour room temperature stability for convenience and improved productivity through automation
• Multiplex up to four targets in one reaction, saving time and resources
• Built-in tolerance for common PCR inhibitors enables robust performance even with challenging samples

A master mix optimized for dPCR performance

Absolute Q Universal DNA Digital PCR Master Mix represents a breakthrough in dPCR technology. This highly optimized master mix is specifically designed to maximize dPCR performance when used with the QuantStudio Absolute Q instrument and MAP16 plates. The master mix expands application possibilities by seamlessly working with predesigned TaqMan Assays. This eliminates the need to design custom assays for your experiments, saving you significant time.

Absolute Q Universal DNA Digital PCR Master Mix has been tested with a range of predesigned TaqMan Assays covering the following applications:

• Gene expression analysis**
• Viral vector quantification
• Mutation detection
• SNP genotyping
• Copy number variation analysis

Seamless workflow from qPCR to dPCR

Absolute Q Universal DNA Digital PCR Master Mix offers the convenience of using the same predesigned TaqMan Assay without modifications for both qPCR and dPCR, providing a straightforward and efficient solution for nucleic acid quantification. This minimizes the need for extensive optimization when transitioning your assays to a dPCR format. The performance of TaqMan Assays is guaranteed when used as directed, giving you confidence in your results.

Higher multiplexing and automation

You can further improve the efficiency of your dPCR workflow by multiplexing up to four targets in a single reaction. The 72-hour benchtop stability also enables extended walkaway time and automation. This allows you to achieve a higher throughput with less hands-on time.

Simple and consistent dPCR procedure

Running your experiments with Absolute Q Universal DNA Digital PCR Master Mix is straightforward. Simply select a predesigned TaqMan Assay, combine it with the master mix and your DNA sample, load into the MAP16 plate, and insert the plate into the QuantStudio Absolute Q instrument. The master mix is optimized to work seamlessly with the QuantStudio Absolute Q system to deliver sensitive and precise dPCR results.

Optimized for storage convenience and shelf life

For improved convenience, Absolute Q DNA Digital PCR Universal Master Mix is stored at 4°C, and it has a two-year shelf life from the date of manufacture.

* We guarantee that all our TaqMan Assays will enable you to obtain data you can trust, or we'll replace them at no cost or give you a credit for the assay purchase price.

** We recommend using cDNA generated using SuperScript IV VILO Master Mix for optimal results.