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  5. STEMCELL Technologies ArciTect™ tracrRNA Kit

STEMCELL Technologies ArciTect™ tracrRNA Kit

Code produit. 17109311
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Quantité:
Kit de 10 nmol
Kit de 20 nmol
Kit de 5 nmol
Conditionnement:
1 kit
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Code produit. Quantité unitSize
17109311 Kit de 20 nmol -
17189301 Kit de 5 nmol 1 kit
17199301 Kit de 10 nmol -
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Code produit. 17109311 Fournisseur STEMCELL Technologies Code fournisseur 76018

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Description

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

  • ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing
  • ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation
  • It is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation
  • It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency
  • The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA
  • ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2 nmol (Catalog #76010), 10 nmol (Catalog #76011), and 20 nmol (Catalog #76012) sizes
  • ArciTect™ Cas9 Nuclease is also available separately (Catalog #76001)

Specifications

Type de cellule Pluripotent Stem Cells
Quantité Kit de 20 nmol
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