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AcroBiosystems CytoPak GMP Human IL-2 Protein

Code produit. 31452286
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Quantité:
15 x 106 IU
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Code produit. 31452286

Marque: AcroBiosystems GMPL02H14GB0115×10^6IU

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Description

Recombinant Protein

Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against self antigens, which could result in autoimmunity. T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

Spécification

Numéro d’adhésion Uniprot
Caractérisation IL-2 Structure This protein carries no tag. The protein has a calculated MW of 15.4 kDa. The protein migrates as 16 kDa±2 kDa when calibrated against under reducing (R) condition (SDS-PAGE).
Format Liquid
Formule Supplied as 0.2 μm filtered solution in phosphate with protectants. Contact us for customized product form or formulation.
Test du contrôle qualité <0.5 ng/μg of protein tested by ELISA.∣<0.1 ng/μg of protein tested by qPCR.∣Less than 5.0 EU/mL, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Quantité 15 x 106 IU
Source CytoPak GMP Human IL-2 Protein (GMP-L02H14GB01) is expressed from E. coli cells. It contains AA Ala 21 - Thr 153 (Accession # ). Predicted N-terminus: Met
Activité biologique CytoPak GMP Human IL-2 Protein (Cat. No. GMP-L02H14GB01) stimulates proliferation of CTLL-2 cells. The specific activity of CytoPak GMP Human IL-2 Protein is ≥1.20 x 10^7 IU/mg, which is calibrated against human Interleukin-2 China National Standard (NIFDC code: 270008) (QC tested). China National Institutes for Food and Drug Control (NIFDC) Standard was prepared and calibrated against human IL-2 WHO International Standard (NIBSC code: 86/500) by NIFDC.∣Human PBMCs were cultured with lyophilized GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) or CytoPak GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14GB01) with CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems) for two weeks. No significant differences were observed in cell viability or proliferation between lyophilized and CytoPak IL-2. Data is the average of 3 independent experiments, error bars ±SD.∣Human PBMCs were cultured with lyophilized GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) or CytoPak GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14GB01) with CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems) for two weeks. No significant differences were observed in the ratio of CD3+ or CD3+ CD4/CD8 positive cells between lyophilized and CytoPak IL-2. Data is the average of 3 independent experiments, error bars ±SD.∣The Cell based assay shows that CytoPak GMP Human IL-2 Protein (Cat. No. GMP-L02H14GB01) is stable at 25°C for 65 hours.∣The Cell based assay shows that CytoPak GMP Human IL-2 Protein (Cat. No. GMP-L02H14GB01) is stable after freezing and thawing 3 times.∣Consistent specific activity (IU/mg) is maintained across lyophilized IL-2 (Cat. No. GMP-L02H14), liquid IL-2 (Cat. No. GMP-L02H14F002), and Cytopak IL-2 (Cat. No. GMP-L02H14GB01).
Espèces Human
Stérilité Sterile
Contenu et stockage -70°C for 24 months; Can be stored up to 1 month at -20°C; Can be stored up to 1 week at 2°C to 8°C. Upon receipt, store it immediately at -70°C or lower for long term storage.
Niveau d’endotoxines Less than 5.0 EU/mL, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Conditions d’expédition This product is supplied and shipped with dry ice, please inquire the shipping cost.
Pureté ou qualité 0.95
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