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STEMCELL Technologies EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Description
- This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh peripheral blood mononuclear cells (PBMCs) or leukapheresis samples
- First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™
- Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion
- The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications
- An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies
- Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands
Spécification
Spécification
| Type de cellule | T Cells, T Cells, CD4+, T Cells, Regulatory |
| À utiliser avec (application) | Cell Isolation |
| Quantité | 1 x 109 cellules |
Nom du produit
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