Gibco™ Expi293™ PRO Cells

Attributes of Expi293 PRO Cells include:

• Suspension culture cells pre-adapted to Expi293 PRO Expression Medium
• Higher protein expression levels than other HEK 293 cells adapted to Expi293 PRO Expression Medium
• More rapid cell growth and higher culture viabilities than standard suspension-adapted HEK 293 cells
• Consistent transient expression performance for 40 passages beyond thaw

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in Expi293 PRO Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 PRO Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.

The cells are typically grown in flasks on a shaker platform in a humidified, CO₂ cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2–7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 4–7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.