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Gibco™ Milieu de base d’Eagle (BME)

Code produit 15470604 Tous les produits Gibco Produits
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Quantité:
500 mL
Conditionnement:
500mL
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15470604 500 mL 500mL
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Product Code. 15470604 Supplier Gibco™ Supplier No. 21010046
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Supplémentation

Le milieu de culture de base Eagle (BME) est un milieu basal synthétique communément utilisé pour favoriser la croissance de nombreuses cellules de mammifères différentes.

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

This BME is manufactured as follows:

  • With: Phenol Red
  • Without: L-glutamine, HEPES

The complete formulation is available.

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

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Conditions d’expédition : Température ambiante

TRUSTED_SUSTAINABILITY

Specifications

Lignée cellulaire HeLa, fibroblastes de souris, WI-38 et MRC-5
Classification Aucun composant d’origine animale
Concentration 1 X
Forme Liquide
Type de produit Milieu de base d’Eagle (BME)
Stérilité Stérilisation par filtration
Avec additifs Faible teneur en glucose, Rouge de phénol
Sans additif Sans glutamine, Sans HEPES, Sans pyruvate de sodium
Qualité de fabrication Conforme aux BPFa en vertu de la norme ISO 13485
Gamme de produits Gibco
Quantité 500 mL
Conditions d’expédition Température ambiante
Contenu et stockage Conditions de stockage : 2 à 8°C. Tenir à l’abri de la lumière
Conditions d’expédition : Température ambiante
Durée de conservation : 12 mois à compter de la date de fabrication
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À des fins de recherche ou de commercialisation avancée Non destiné aux diagnostics ou à l’administration directe à des humains ou des animaux.

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