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Description
Requires
Supplémentation
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.
This BME is manufactured as follows:
- With: Phenol Red
- Without: L-glutamine, HEPES
The complete formulation is available.
BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.
BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Conditions d’expédition : Température ambiante
Specifications
Specifications
| Lignée cellulaire | HeLa, fibroblastes de souris, WI-38 et MRC-5 |
| Classification | Aucun composant d’origine animale |
| Concentration | 1 X |
| Forme | Liquide |
| Type de produit | Milieu de base d’Eagle (BME) |
| Stérilité | Stérilisation par filtration |
| Avec additifs | Faible teneur en glucose, Rouge de phénol |
| Sans additif | Sans glutamine, Sans HEPES, Sans pyruvate de sodium |
| Qualité de fabrication | Conforme aux BPFa en vertu de la norme ISO 13485 |
| Gamme de produits | Gibco |
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À des fins de recherche ou de commercialisation avancée Non destiné aux diagnostics ou à l’administration directe à des humains ou des animaux.
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