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AcroBiosystems GMP Human BMP-4 / BMP2B Protein

Code produit 31453212
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Code produit Quantité
31453212 10 mg
31453197 50 μg
31451640 1 mg
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Code produit 31453212 Fournisseur AcroBiosystems Code fournisseur GMPBM4H3610mg

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Description

Recombinant Protein

Bone Morphogenetic Protein 4 (BMP4) is a member of growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. BMP-4 can acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

Spécification

Numéro d’adhésion Uniprot
Caractérisation BMP-4 Structure This protein carries no tag. The protein has a calculated MW of 13.1 kDa. The protein migrates as 21 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE) due to glycosylation.
Format Powder-GMP
Formule Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH 2.2 with protectants. Contact us for customized product form or formulation.
Test du contrôle qualité <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Quantité 10 mg
Source GMP Human BMP-4 Protein (GMP-BM4H36) is expressed from CHO cells. It contains AA Ser 293 - Arg 408 (Accession # ). Predicted N-terminus: Ser 293 Request for sequence
Activité biologique GMP Human BMP-4 Protein (Cat. No. GMP-BM4H36) stimulates Human BMP (Luc) HEK293 Reporter cells. The typical EC50 for this effect is 2.563 ng/mL (QC tested).∣GMP Human BMP-4 Protein (Cat. No. GMP-BM4H36) could promote the differentiation of iPSCs into hematopoietic stem and progenitor cells in a feeder-free system at Day 14, with high expression of CD34+ CD45+. GMP Human BMP-4 Protein (Cat. No. GMP-BM4H36) holds a similar performance with Human BMP-4 Protein, premium grade (Cat. No. BM4-H5317).∣The Cell based assay shows that GMP Human BMP-4 Protein (Cat. No. GMP-BM4H36) is stable at 37°C for 7 hours.∣The Cell based assay shows that GMP Human BMP-4 Protein (Cat. No. GMP-BM4H36) is stable at 4°C for 180 days.∣The Cell based assay shows that GMP Human BMP-4 Protein (Cat. No. GMP-BM4H36) is stable after freezing and thawing 3 times.
Espèces Human
Stérilité Sterile
Contenu et stockage -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. Upon receipt, store it immediately at -20°C or lower for long term storage.
Niveau d’endotoxines Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Conditions d’expédition This product is supplied and shipped with blue ice, please inquire the shipping cost.
Pureté ou qualité 0.95
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