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AcroBiosystems GMP Human IL-3 Protein
Description
Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human and murine IL-3 share low homology and it does not show activity on murine cells.
Spécification
Spécification
| Numéro d’adhésion | Uniprot |
| Caractérisation | IL-3 Structure This protein carries no tag. The protein has a calculated MW of 15.2 kDa. The protein migrates as 14 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE). |
| Format | Powder-GMP |
| Formule | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation. |
| Test du contrôle qualité | <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative |
| Quantité | 1 mg |
| Source | GMP Human IL-3 Protein (GMP-L03H18) is expressed from E. coli cells. It contains AA Ala 20 - Phe 152 (Accession # ). Request for sequence |
| Activité biologique | GMP Human IL-3 Protein (Cat. No. GMP-L03H18) stimulates proliferation of TF-1 cells. The specific activity of GMP Human IL-3 Protein is >1.00x10^6 IU/mg, which is calibrated against human IL-3 WHO International Standard (NIBSC code: 91/510) (QC tested).∣The activity of GMP Human IL-3 (Cat. No. GMP-L03H18) was higher than other competing products.∣GMP Human SCF Protein (Cat. No. GMP-SCFH25), Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human IL-3 Protein (Cat. No. GMP-L03H18) and GMP Human IL-6 Protein (Cat. No. GMP-L06H27) could support the rapid cell expansion and good cell viability of CD34+ hematopoietic stem cells.∣The Cell based assay shows that GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable at 37°C for 24 hours.∣The Cell based assay shows that GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable at 4°C for 180 days.∣The Cell based assay shows that GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable after freezing and thawing 3 times.∣The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-3.∣MALDI-TOF analysis of GMP Human IL-3 Protein (Cat. No. GMP-L03H18). The labeled peaks at 15209.9 Da and 15080.7 Da correspond to the calculated molecular mass with the N-terminal Met (15213 Da), and without the N-terminal Met (15081 Da), respectively. The minor peak following the major peak is a matrix-associated artifact of the MALDI-TOF (Routinely tested). |
| Espèces | Human |
| Stérilité | Sterile |
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Nom du produit
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