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AcroBiosystems GMP Human IL-6 Protein

Code produit 31453643
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31453643 1 mg
31452888 10 mg
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Code produit 31453643 Fournisseur AcroBiosystems Code fournisseur GMPL06H271mg

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Recombinant Protein

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Spécification

Numéro d’adhésion Uniprot
Caractérisation IL-6 Structure This protein carries no tag. The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-29 kDa when calibrated against under reducing (R) condition (SDS-PAGE) due to glycosylation.
Format Powder-GMP
Formule Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation.
Test du contrôle qualité <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Quantité 1 mg
Source GMP Human IL-6 Protein (GMP-L06H27) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # ). Predicted N-terminus: Val 30 Request for sequence
Activité biologique GMP Human IL-6 Protein (Cat. No. GMP-L06H27) stimulates proliferation of TF-1 human erythroleukemic cell line. The specific activity of GMP Human IL-6 Protein is >1.00x10^8 IU/mg, which is calibrated against human IL-6 WHO International Standard (NIBSC code: 21/308) (QC tested).∣The activity of GMP Human IL-6 Protein (Cat. No. GMP-L06H27) was higher than other competing products.∣GMP Human SCF Protein (Cat. No. GMP-SCFH25), Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human IL-3 Protein (Cat. No. GMP-L03H18) and GMP Human IL-6 Protein (Cat. No. GMP-L06H27) could support the rapid cell expansion and good cell viability of CD34+ hematopoietic stem cells.∣The Cell based assay shows that GMP Human IL-6 Protein (Cat. No. GMP-L06H27) is stable at 37 °C for 24 hours.∣The Cell based assay shows that GMP Human IL-6 Protein (Cat. No. GMP-L06H27) is stable after freezing and thawing 3 times.∣The Cell based assay shows that GMP Human IL-6 Protein (Cat. No. GMP-L06H27) is stable at 4°C for 6 months.∣The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-6.
Espèces Human
Stérilité Sterile
Contenu et stockage -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. Upon receipt, store it immediately at -20°C or lower for long term storage.
Niveau d’endotoxines Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Conditions d’expédition This product is supplied and shipped with blue ice, please inquire the shipping cost.
Activité spécifique The activity of GMP Human IL-6 Protein (Cat. No. GMP-L06H27) was higher than other competing products.
Pureté ou qualité 0.95
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