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  5. Invitrogen™ Kit ELISA humain d’adiponectine

Invitrogen™ Kit ELISA humain d’adiponectine

Code produit 15581527
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Quantité:
10 x 96 tests
Conditionnement:
10 pièces
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15581527 10 x 96 tests 10 pièces
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Code produit 15581527 Fournisseur Invitrogen™ Code fournisseur BMS20322TEN

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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Adiponectin
Biotin-Conjugate anti-human Adiponectin monoclonal antibody
Streptavidin-HRP
Human Adiponectin Standard lyophilized, 30ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Kit ELISA sandwich

The Human Adiponectin ELISA quantitates Hu Adiponectin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Adiponectin. Principle of the method The Human Adiponectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.
TRUSTED_SUSTAINABILITY

Spécification

Numéro d’adhésion Q15848
Plage de dosage 0,23-15 ng/ml
Sensibilité du dosage 0,012ng
Conjugué HRP
Méthode de détection Colorimétrique
Format du kit d’immunoessai Sandwich ELISA Kit
Type de produit Kit ELISA
Type d’échantillon Sérum, Surnageant, Supernatant
Espèces cibles Humaine
À utiliser avec (application) ELISA
À utiliser avec (équipement) Lecteur de microplaques colorimétriques
Alias de gène 30 kDa adipocyte complement-related protein; 30kDa; Acdc; Acrp30; Ad; adipo; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; adiponectin; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPIOQ; ADPN; Apm1; APM-1; APN; GBP28; Gelatin-binding protein; gelatin-binding protein 2830 kDa adipocyte complement-related protein; 30kDa; Acdc; Acrp30; Ad; adipo; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; adiponectin; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPIOQ; ADPN; Apm1; APM-1; APN; GBP28; Gelatin-binding protein; gelatin-binding protein 28; ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
Identification génétique (Entrez) 9370
Symbole de gène(s) ADIPOQ
Coefficient de variation inter-dosage 3.1%
Coefficient de variation intra-dosage 4.2%
Contenus des kits Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer Concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Marqueur ou colorant HRP
Quantité 10 x 96 tests
État réglementaire RUO
Volume d’échantillon Plasma, 10 μl ; sérum, 10 μl ; surnageant, 10 μl
Conditions de stockage 2°C à 8°C
Synonyme ACDC, apM 1, GBP-28, ADIPOQ
Cible Humaine
Heure du test 1 heures 20 min
Durée totale du dosage 3 h 30 min
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