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Gibco™ Human HGF Recombinant Protein, PeproTech®

Code produit 17860763
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Code produit Quantité unitSize
17860763 100 μg 100µg
17861023 1 mg 1mg
17820263 250 μg 250µg
17829892 25 μg 25µg
17891223 500 μg 500µg
17870553 5 μg 5µg
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Code produit 17860763 Fournisseur Gibco™ Code fournisseur 10039H100UG

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Recombinant Protein

Recombinant Human HGF, sourced from HEK293 cells, is a 79.4 kDa polypeptide consisting of 695 amino acid residues. As a result of glycosylation, Recombinant Human HGF migrates with an apparent molecular mass of approximately 68-85 kDa by SDS-PAGE gel, under non-reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
TRUSTED_SUSTAINABILITY

Spécification

Numéro d’adhésion P14210
À utiliser avec (application) Functional Assay
Formule protein with no preservative
Identification génétique (Entrez) 3082
Poids moléculaire 79.4 kDa
Nom Human HGF
Quantité 100 μg
Source HEK293 cells
État réglementaire RUO
Concentration en endotoxines <1 EU/ μg
Alias de gène C230052L06Rik; DFNB39; Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; hepatopoeitin-A; HepatopoietinA; hepatopoietin-A; Hgf; HGF/SF; HGFB; HPTA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; NK1; NK2; Scatter factor; scatter factor); SF; SF/HGF
Nom usuel HGF
Symbole de gène(s) Hgf
Activité biologique Determined by the dose-dependent stimulation of the proliferation of monkey 4 MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml
Conjugué Unconjugated
Recombinant Recombinant
Séquence Alpha chain: KRRNTIHEFK KSAKTTLIKI DPALKIKTKK VNTADQCANR CTRNKGLPFT CKAFVFDKAR KQCLWFPFNS MSSGVKKEFG HEFDLYENKD YIRNCIIGKG RSYKGTVSIT KSGIKCQPWS SMIPHEHSFL PSSYRGKDLQ ENYCRNPRGE EGGPWCFTSN PEVRYEVCDI PQCSEVECMT CNGESYRGLM DHTESGKICQ RWDHQTPHRH KFLPERYPDK GFDDNYCRNP DGQPRPWCYT LDPHTRWEYC AIKTCADNTM NDTDVPLETT ECIQGQGEGY RGTVNTIWNG IPCQRWDSQY PHEHDMTPEN FKCKDLRENY CRNPDGSESP WCFTTDPNIR VGYCSQIPNC DMSHGQDCYR GNGKNYMGNL SQTRSGLTCS MWDKNMEDLH RHIFWEPDAS KLNENYCRNP DDDAHGPWCY TGNPLIPWDY CPISRCEGDT TPTIVNLDHP VISCAKTKQL R Beta chain: VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS
Contenu et stockage -20°C
Système d’expression HEK293 cells
Forme Lyophilized
Pureté ou qualité ≥ 95% by SDS-PAGE gel and HPLC analyses.
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