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Invitrogen™ Human IL-6 Rapid ELISA Kit

Code produit. 30828487
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Quantité:
96 dosages
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Code produit. Quantité
30828487 96 dosages
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Code produit. 30828487 Fournisseur Invitrogen™ Code fournisseur EELR002

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Description

ELISA

The Human Interleukin 6 (IL 6) ELISA quantitates IL 6 in serum and plasma. Principle of the method The Human IL 6 solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody. A substrate solution is added that reacts with the enzyme antibody target complex to produce a measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Spécification

Numéro d’adhésion P05231
Plage de dosage 3.13-200 pg/mL
Sensibilité du dosage 1.02 pg/mL
Conjugué HRP
Format du kit d’immunoessai Coated ELISA Kit
Type de produit ELISA
Type d’échantillon Plasma, Serum
Espèces cibles Human
À utiliser avec (équipement) Absorbance Microplate Reader
Alias de gène B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6;
Identification génétique (Entrez) 3569
Symbole de gène(s) Il6
Coefficient de variation inter-dosage <10%
Coefficient de variation intra-dosage <10%
Contenus des kits Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Marqueur ou colorant HRP
Quantité 96 dosages
État réglementaire RUO
Volume d’échantillon Plasma 50 μL, Serum 50 μL
Conditions d’expédition Wet or Dry Ice
Conditions de stockage Refer to product documentation for component specific storage temperature.
Cible IL-6
Heure du test 15 min
Durée totale du dosage 1 hr 30 min
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