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Invitrogen™ Human IL-6 ELISA Kit

Product Code. 10390964
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Quantité:
96 dosages
Unit Size:
1 kit
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Product Code. Quantité Unit Size
10390964 96 dosages 1 kit
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Product Code. 10390964 Supplier Invitrogen™ Supplier No. EH2IL6
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Description

ELISA

The Human Interleukin-6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Specifications

Numéro d’adhésion P05231
Plage de dosage 10,24-400 pg/ml
Sensibilité du dosage <1 pg/ml
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Description Kit ELISA pour IL-6 humain
Conjugué Biotine
Format du kit d’immunoessai ELISA colorimétrique (substrat TMB) dans la plaque de bandelette à 96 puits transparents
Type de produit Kit ELISA
Famille de protéines Cytokines & Receptors
Type d’échantillon Sérum, Surnageant, Surnageant, Urine
Spécificité Aucune réaction croisée importante avec IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF humains ou IL-6 de souris
Suffisant pour 96 puits de microplaques
Espèces cibles Humaine
À utiliser avec (équipement) Lecteur de microplaques colorimétriques
Alias de gène B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Identification génétique (Entrez) 3569
Symbole de gène(s) BSF-2,BSF2,CDF,HGF,HSF,IFN-bêta-2,IFNB2,IL-6
Coefficient de variation inter-dosage < 10 %
Coefficient de variation intra-dosage < 10 %
Contenus des kits Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection biotinylé, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives, Concentré de streptavidine-HRP, 75 µl
Marqueur ou colorant HRP
Mesure Absorbance à 450 nm moins absorbance à 550 nm
Précision CV intra-dosage <10 % ; CV inter-dosage <10 %
Quantité 96 dosages
État réglementaire RUO
Volume d’échantillon Plasma, 50 μl ; Sérum, 50 μl ; Surnageant, 50 μl ; Urine, 50 μl
Conditions de stockage 2°C à 8°C
Cible Humaine
Heure du test 1 heures 20 min
Durée totale du dosage 3 h
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Safety and Handling

compliance-icons
Product Identifier
  • Human IL-6 ELISA Kit
 Signal Word
  • Attention
 Hazard Category
  • Sensibilisation cutanée Catégorie 1
 Hazard Statement
  • H317-Peut provoquer une allergie cutanée.
 Precautionary Statement
  • P261-Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/ aérosols.
  • P272-Les vêtements de travail contaminés ne devraient pas sortir du lieu de travail.
  • P280-Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/ du visage.
  • P302+P352-EN CAS DE CONTACT AVEC LA PEAU: Laver abondamment à l'eau/.
  • P333+P313-En cas d'irritation ou d'éruption cutanée: consulter un médecin.
  • P362+P364-Enlever les vêtements contaminés et les laver avant réutilisation.
  • P501b-Éliminer le contenu/récipient conformément à la réglementation locale/régionale/nationale/internationale.
 Supplemental information
  • MIXTURE LIST-Contain: 2-methyl-3(2H)-isothiazolone
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