Berthold Technologies LightCompass™ Professional Data Acquisition, Analysis and Reporting Software

Workflow

Protocol setup

• Create or open a plate layout.
• Open a preloaded protocol (includes instrument settings, analysis functions, and export/print settings), use it as a template, or build a new one with the protocol wizard.
• Create fully custom protocols if detailed control is required.

Measurement capabilities

• Reagent dispensing and incubation steps
• Endpoint
• Kinetics (including long-term/repeated measures over several days)
• Wavelength scan
• Area scan
• Multiple measurement modes within a single protocol

Data analysis

• Standard curves with curve-fitting options: linear, polynomial, 4PL, smoothed cubic spline, others
• Threshold-based classification for qualitative assays
• Custom formulas
• Quantitative and qualitative analysis tools
• Outlier correction
• Unlimited calculation matrices
• Dilution factor calculation
• Customized analysis report generation

Data handling and compliance

• Print or export results to Excel or LIMS
• User accounts with configurable levels, hierarchies, and password protection
• Active Directory integration
• Audit trail with automated log files
• QC/validation settings with pass/fail criteria
• Secure data storage (local file system or remote SQL server)
• FDA 21 CFR Part 11 and EU GMP Annex 11 compliance
• Control history for validation
• Assay lot number verification

Sample and plate management

• Sample identification (manual or barcode)
• Plate Organizer (sample ID definition, preparation)
• Automated filename generation
• Parameter file selection by barcode
• Automation Server support

Applications

• ELISA
• Nucleic acid and protein quantification
• Reporter gene assays
• Dose-response assays
• Kinetic assays
• BRET
• TR-FRET and HTRF™