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Invitrogen™ Lyo-ready Bst DNA Polymerase
Description
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.
Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs
Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)
Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.
Spécification
Spécification
| Type de produit | Stand-alone enzyme |
| À utiliser avec (application) | LAMP, RT-LAMP |
| Polymérase | Lyo-ready Bst DNA Polymerase |
| Quantité | 1 200 U |
| Contenu et stockage | • Lyo-ready Bst DNA Polymerase (200 μL at 6 U/μL) |
| Nbre de réactions | 200 Reactions |
| Température de réaction optimale | 65°C |
| Conditions d’expédition | Dry Ice |
| Concentration | 6 U/μl |
| Temps de réaction | ≤10 minutes |
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
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