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  6. Invitrogen™ Lyo-ready Bst DNA Polymerase

Invitrogen™ Lyo-ready Bst DNA Polymerase

Code produit 17885207
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Quantité:
1 200 U
6 000 U
Concentration:
40 U/μl
6 U/μl
Conditionnement:
1200 unités
6000 unités
3 product options available for selection
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Code produit Quantité Concentration unitSize
17885207 1 200 U 40 U/μl 1200 unités
17815017 1 200 U 6 U/μl 1200 unités
17805217 6 000 U 6 U/μl 6000 unités
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This item is not returnable. View return policy
Pour bénéficier de la remise, les clients doivent acheter 2 unités du même produit au prix catalogue dans une seule commande afin d’obtenir une réduction de 30 %. Il n’y a pas de limite au nombre de lots de 2 que les clients peuvent acheter.
Product Code. 17885207 Supplier Invitrogen™ Supplier No. A56657

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51.45 EUR valable jusqu'au 2026-05-15
MEILLEUR PRIX promo! Utilisez le code promo "27974" pour bénéficier de cette offre.
This item is not returnable. View return policy
Pour bénéficier de la remise, les clients doivent acheter 2 unités du même produit au prix catalogue dans une seule commande afin d’obtenir une réduction de 30 %. Il n’y a pas de limite au nombre de lots de 2 que les clients peuvent acheter.

Description

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.

Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs

Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)

Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.

TRUSTED_SUSTAINABILITY

Specifications

Type de produit Stand-alone enzyme
À utiliser avec (application) LAMP, RT-LAMP
Polymérase Lyo-ready Bst DNA Polymerase
Quantité 1 200 U
Contenu et stockage

• Lyo-ready Bst DNA Polymerase (30 μL at 40 U/μL)
• 10X Bst Reaction Buffer (1 mL)
• 200 mM MgCl2 (1 mL)
• 5 M Betaine (1 mL)

Store at –15 to –25°C.
Nbre de réactions 200 Reactions
Température de réaction optimale 65°C
Conditions d’expédition Dry Ice
Concentration 40 U/μl
Temps de réaction ≤10 minutes

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

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