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  6. Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Code produit 30245142
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Quantité:
100 réactions
120 μg
1 600 μg
2 000 μl
Type de produit:
Kit Lyo-ready RPA
Protéine T4 Gene 32 Lyo-ready
Protéine T4 UvsX Lyo-ready
Protéine UvsY T4 Lyo-ready
Tampon de réaction
Conditionnement:
100 réactions
120µg
1600µg
2000µL
5 product options available for selection
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Code produit Quantité Type de produit unitSize
30245142 100 réactions Kit Lyo-ready RPA 100 réactions
30245335 1 600 μg Protéine T4 Gene 32 Lyo-ready 1600µg
30245214 120 μg Protéine T4 UvsX Lyo-ready 120µg
30245152 120 μg Protéine UvsY T4 Lyo-ready 120µg
30245027 2 000 μl Tampon de réaction 2000µL
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This item is not returnable. View return policy
Pour que la promotion s’applique, le client doit acheter trois fois le même produit au prix catalogue sur la même commande pour avoir une remise de 33.33%. Il n’y a pas de limite d’achat pour un multiple de 3 produits. PCODE: 27968
Product Code. 30245142 Supplier Invitrogen™ Supplier No. A72127

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This item is not returnable. View return policy
Pour que la promotion s’applique, le client doit acheter trois fois le même produit au prix catalogue sur la même commande pour avoir une remise de 33.33%. Il n’y a pas de limite d’achat pour un multiple de 3 produits. PCODE: 27968

Description

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs
TRUSTED_SUSTAINABILITY

Specifications

Type de produit Kit Lyo-ready RPA
Forme Liquid
À utiliser avec (application) RPA, RT-RPA
Polymérase Lyo-ready Bst DNA Polymerase
Quantité 100 réactions
Catégorie de recherche Nucleic Acid Amplification
Contenu et stockage

• Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
• Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
• Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
• Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
• 2X RPA Reaction Buffer, 1000 μL
• 280 mM MgCl2, 100 μL
• 10 mM dNTP Mix, 50 μL

Store at -25°C to -15°C.
Température de réaction optimale 42°C
Transcriptase inverse SuperScript IV Reverse Transcriptase
Conditions d’expédition Dry Ice
Méthode de détection End point, real-time, lateral flow
Temps de réaction Up to 20 min.
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Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

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