Learn More
Description
MabCaptureC Affinity Matrix is an agarose-based, high-binding-capacity Protein A chromatography resin with a rProtein A ligand produced in yeast. The engineered rProtein A binds at the CH2-CH3 interface of the Fc-region of IgGs and shows cross binding to the VH3 domain of IgGs.
MabCaptureC™ Affinity Matrix is suitable for the purification of monoclonal antibodies.The rProtein A ligand is immobilized on a highly cross-linked and uniform-sized agarose-based bead with an average particle size of 75 μm. The uniform particle size delivers superior performance characteristics compared to traditional Protein A resins.
Features of the MabCaptureC™ Affinity Matrix include:
- High dynamic binding capacity for mAbs of >50 g/L at 4.8 min residence time
- Purification of mAbs and a subpopulation of scFv's and Fab fragments containing VH3 binding domains
- Clean and sanitize with standard sodium hydroxide agents for chromatography products
- Excellent scalability
- Non-animal derived
Main characteristics
- Matrix: agarose-based, epoxide activated
- Average particle size: 75 ± 10 μm
- Ligand: recombinant C domain of Protein A
- Ligand coupling method: epoxide coupling
- Binding capacity: >50 g IgG/L resin at 4.8 min residence time and 10% breakthrough
- Elution conditions: 0.1 M glycine pH 3.0, or 20–100 mM sodium citrate or acetate, pH 3.0
- Cleaning conditions: 0.2 M sodium hydroxide for 15 minutes every cycle
- Operating pressure: ≤3 bar
- Pressure flow: >500 cm/h at 3 bar in a 2.6 x 20 cm column
- Formulation buffer: 20% (v/v) ethanol
For process development and resin screening, MabCaptureC™ is also available in the following formats:
- MabCaptureC™ MiniChrom Column, 1 mL (Cat. No. 5943662001)
- MabCaptureC™ MiniChrom Column for process development, 5 mL (5943662005)
- MabCaptureC™ RoboColumn, 200 μL (5943662200)
- MabCaptureC™ RoboColumn, 600 μL (5943662600)
Spécification
Spécification
| Type de produit | Affinity Matrix |
| Capacité de liaison | >50 g IgG/L |
| Ligand | Engineered rProtein A |
| Matrice | Agarose-based, epoxide-activated |
| Granulométrie | 60–80 μm |
| Cible | CH2-CH3 interface Fc-region |
| Formule | Suspension in 20% ethanol |
| À utiliser avec (application) | Monoclonal antibody purification |
| Quantité | 250 ml |
| Forme | Agarose Bead |
À des fins de recherche ou de commercialisation avancée Non destiné aux diagnostics ou à l’administration directe à des humains ou des animaux.
En cliquant sur Soumettre, vous reconnaissez que vous pouvez être contacté par Fisher Scientific au sujet des informations que vous avez fournies dans ce formulaire. Nous ne partagerons pas vos informations à d'autres fins. Toutes les informations de contact fournies seront également conservées conformément à notre politique de confidentialité. Politique de confidentialité.