Invitrogen™ Mouse IFN-gamma (ELISA Standard) Recombinant Protein

Recombinant Protein

Description: Mouse IFN-gamma is a ~20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-gamma, in synergy with other cytokines such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity. Applications Reported: Recombinant mouse IFN gamma is biologically active. The intended use of the Recombinant Mouse IFN gamma Single-Use ELISA RSG Standard is as a standard for mouse IFN gamma Ready-SET-Go! ELISA and mouse IFN gamma matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard described below. Instructions for use (XMG1.2/R4-6A2 assays): For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 μL of standard solution to 10 mL of assay diluent to prepare the top standard solution (2000 pg/mL).For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 μL of standard solution to 10 mL of assay diluent to prepare the top standard solution (2000 pg/mL). Instructions for use (AN-18/R4-6A2 assays): For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 μL of standard solution to 10 mL of assay diluent to prepare 2000 pg/mL solution. Mix thoroughly.