Invitrogen™ Novex™ Tris-Glycine Plus Midi Protein Gels, 4 to 20%, 1.0 mm, WedgeWell™ format

Novex Tris-Glycine Plus WedgeWell format midi protein gels are improved polyacrylamide gels based on the traditional Laemmli protein chemistry, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus WedgeWell format midi gels are a 1.0-mm thick, wider format (8 x 13 cm) gel for higher throughput electrophoresis of protein samples.

Product features

• Flexibility—use for native and denaturing protein assays
• Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes
• Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Run your proteins in native or denatured form

Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in a native or denatured state. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC26754). For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer (LC2673) and Novex Tris-Glycine Native Running Buffer (LC2672). The gels can be run in the SureLock Tandem Midi Gel Tank (STM1001) or XCell4 SureLock Midi-Cell (WR0100).

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer (LC3675) when performing traditional wet transfer with the SureLock Tandem Midi Blot Module (STM2001). Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001).

Convenient to use

Novex Tris-Glycine Plus WedgeWell format gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, which makes sample loading easier.