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Beskrivning
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
Comprend :
Tampon Phusion HF 5x, solution de MgCl2 de 50 mM, DMSO à 100 %.
Specifikationer
Specifikationer
| Nom chimique ou matériau | HF Buffer Pack |
| Couleur | Colorless |
| Description | Boîte de tampons Phusion™ HF |
| À utiliser avec (application) | PCR standard |
| Quantité | 6 mL |
| Comprend | Le pack de tampon Phusion HF (F-518L) comprend : • Tampon Phusion HF 5X (tampon 1X fournissant 1,5 mM de MgCl2) • 50 mM de MgCl2 • DMSO Conserver à -20°C. |
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
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