Learn More
Invitrogen™ Ensemble de signaux de sécrétion PichiaPink™
Description
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors.
Optimize Secretion of Your Expressed Protein
By adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastoris secretes few native proteins, so the bulk of the protein in the media will be yours.
Each DNA sequence encodes a different secretion signal. You can test each secretion signal sequence to find the one that works best for your protein.
The PichiaPink™ Secretion Signal Set comes with 8 different secretion signals including:
- α-mating factor pre-sequence from Saccharomyces cerevisiae
- α-amylase signal sequence from Aspergillus niger
- Glucoamylase signal sequence from Aspergillus awamori
- Serum albumin signal sequence from Homo sapiens
- Inulinase signal sequence from Kluyveromcyes maxianus
- Invertase signal sequence from Saccharomyces cerevisiae
- Killer protein signal sequence from Saccharomyces cerevisiae
- Lysozyme signal sequence from Gallus gallus
Easily Add a Secretion Signal to Your Protein
The DNA fragments in the PichiaPink™ Secretion Signal Set are designed to be easily inserted in front of your gene when using the pPINK-HC or pPINK-LC vectors. You can try different secretion signal sequences to see which one gives optimal secretion of your protein.
Each signal sequence includes the Kozak sequence for the AOX1 gene from Pichia pastoris to ensure efficient translation of your protein.
The secretion signal sequences come as phosphorylated duplex oligomers in 40 pmol aliquots. Resuspend oligomers in 40 μL TE buffer before use.
Why Choose the PichiaPink™ Yeast Expression System?
The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.
For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.
References
1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]
2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]
Spécification
Spécification
| Description | Clonage dans des vecteurs d’expression |
| À utiliser avec (application) | Biologie des protéines, expression des protéines, expression des levures, systèmes d’expression Pichia pastoris, clonage en vecteurs d’expression |
| Type de produit | Kit du signal de sécrétion PichiaPink |
| Quantité | 1 kit |
| Espèces | P. pastoris |
| Souche bactérienne ou de levures | PichiaPink™ |
| Contenu et stockage | L’ensemble de signaux de sécrétion PichiaPink™ comprend 8 séquences de signaux qui sont des duplexes ADN : • Préséquence facteur d’accouplement alpha de S. cerevisiae • Asp. niger alpha Amylase • Signal glucoamylase Asp. awarmori • Signal albumine sérique H. sapien • Préséquence inulinase K. maxianus Inulinase • Séquence tueuse de protéines S.cerevisiae • Signal de séquence invertase S.Cerevisiae invertase • Signal de séquence lysozyme G. gallus Lysozyme Quantité : 40 pmoles Conserver à -20°C |
| Méthode de clonage | Enzyme de restriction / MCS |
| Accélérateur | AOX1 |
| Vector | pPINK-LC, pPINK-HC |
| Afficher plus |
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
En cliquant sur Soumettre, vous reconnaissez que vous pouvez être contacté par Fisher Scientific au sujet des informations que vous avez fournies dans ce formulaire. Nous ne partagerons pas vos informations à d'autres fins. Toutes les informations de contact fournies seront également conservées conformément à notre politique de confidentialité. Politique de confidentialité.