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Rat spleen tissue lysate, Non-denatured; Abnova
Description
- Tissue: Spleen
- Host: Rat
- Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
- Storage buffer: In modified RIPA lysis buffer
Western Blotting, Immunoprecipitation
Spécification
Spécification
| À utiliser avec (application) | Immunoprecipitation, Western Blot |
| Tissue | Spleen |
| Description | Whole tissue lysate (non-denatured) |
| Tampon de lyse | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Méthode de préparation | Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml. |
| Test du contrôle qualité | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quantité | 200 μg |
| Tampon de stockage | In modified RIPA Lysis Buffer. |
| Espèces hôtes | Rat |
| Concentration | 5 mg/mL |
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Nom du produit
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